atcc vr  (ATCC)

Journal: iScience

Article Title: Broad-spectrum antiviral activity of antisense oligonucleotides targeting GBF1 against SARS-CoV-2 and influenza viruses

doi: 10.1016/j.isci.2026.114851

Figure Lengend Snippet: Identification of host factors involved in SARS-CoV-2 and HCoV-229E replication (A) Schematic overview of the siRNA screening. HEK293 A/T cells were seeded in 24-well plates and transfected twice with siRNAs targeting 91 host genes previously identified as being involved in influenza virus replication. Cells were then infected with SARS-CoV-2 (100 plaque-forming unit [PFU]/100 μL) one day after the second transfection. Supernatants were collected at 2 days post-infection (dpi) and titrated by plaque assay. (B) Results of the SARS-CoV-2 siRNA screen. The 91 host factors were divided into four batches, each including a non-targeting siRNA as a negative control (N) and siRNA targeting SARS-CoV-2 nsp12 as a positive control (P). Viral titers were calculated based on the difference between each siRNA and its corresponding negative control. Each dot represents the mean of duplicate wells from a single independent experiment. Data are presented as the mean ± standard deviation (SD) of at least three independent experiments. (C) The seven host factors identified in the SARS-CoV-2 siRNA screen were further examined in HCoV-229E, along with the negative control siRNA (N) and positive control siRNA (P) targeting the HCoV-229E N gene. Following the same method described in (A), MRC-5 cells were infected with HCoV-229E (50 tissue culture infectious dose (TCID 50 )/100 μL). Supernatants were collected at 3 dpi and titrated by TCID 50 assay. Viral titers were calculated based on the difference between each siRNA and the negative control. Each dot represents the mean of duplicate wells from a single independent experiment. Data are presented as the mean ± SD of three independent experiments. (D) Cell viability was measured in duplicate wells across two independent experiments using CellTiter-Glo following siRNA transfection. See also and .

Article Snippet: HCoV-229E , ATCC , VR-740.

Techniques: Transfection, Virus, Infection, Plaque Assay, Negative Control, Positive Control, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Features and In Vitro Assessment of Antiviral Activity of Organic Coatings Doped with Silver-Based Compounds Against Human Coronavirus

doi: 10.3390/ijms262211068

Figure Lengend Snippet: Inverted fluorescence microscopy images of the cell lines utilised for the cultivation of HCoVs-229E, OC43, and NL63. The red frames in figures ( A – C ) denote CPE, which refer to the observable morphological and degenerative alterations occurring 3–7 days post-infection by HCoVs (for instance, clumped cell aggregates or rounded host cells): ( A ) LLC-MK2 cell line infected with HCoV-NL63; ( B ) HCT-8 cell line infected with HCoV-OC43; ( C ) MRC-5 cell line infected with HCoV-229E. The uninfected cell lines are represented as follows: ( D ) LLC-MK2; ( E ) HCT-8; and ( F ) MRC-5.

Article Snippet: For virus stock multiplication, reference strains of HCoV, specifically 229E (ATCC-VR-740) (LGC Standards, Teddington, UK), OC43 (ATCC-VR-1558) (LGC Standards), and NL63 (Małopolskie Centrum Biotechnologii, Kraków, Poland) were cultivated on suitable adherent host cells: HCoV-229E/MRC-5 (Cytion), HCoV-OC43/HCT-8 (Cytion), and NL63/LLC-MK2 (Cytion) by incubating at temperatures between 33 and 35 °C with 5% CO 2 for a duration of 1–2 h to facilitate virus adsorption into the cells.

Techniques: Fluorescence, Microscopy, Infection